Supervisor(s):Chinese Pharmaceutical Association Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association ISSN:0253-2670 CN:12-1108/R
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica.
The journal is included in CA, JST and CSCD.
Objective To prepare the molecularly imprinted polymer (MIPs) of L-tetrahydropalmatine (L-THP) by the molecular imprinting technique and study on solid-phase extraction. Methods Using L-THP as template, methyl acrylic acid (MAA) as functional monmer, and ethylene glycol dimethacrylate (EDMA) as a cross-linking agent to prepare the L-THP-MIPs. A test was conducted to investigate the selectivity and the specificity of solid-phase extraction. Results The experiment showed that the MIPs had the specific adsorption to L-THP, but did not have the specific adsorption to corydaline the structural analogue with L-THP. Conclusion The L-THP-MIPs have a good selectivity and the specificity of L-THP.
Objective To investigate the method for study on the effect of factors on pepsin and trypsin fibrinolytic activity and deactivation of fibrinolytic activity and to eliminate the interference of pepsin and trypsin on the detection of crude protein fibrinolytic activity of Armadillidium vulgare (porcellio plasmin) in order to obtain the proteins or peptides which have the smaller molecular weight but higher titer during the pepsin and trypsin degradation. Methods To study the effect of pepsin and trypsin deactivation on pH value, temperature, metal ions, enzyme inhibitor, surfactant, and responsing fibrinolytic, the fiber fibrin plate assay was usede. The better enzyme deactivation process was obtained and used for studying the effect on the fibrinolytic activity of urokinase, lumbrokinase, and porcellio plasmin. Results All the pH value, temperature, metal ions, enzyme inhibitor, and surfactant have had an impact on pepsin and trypsin fibrinolytic activity. Among them the optimum deactivation of pepsin was pH 6.0–8.0, while the optimum deactivation of trypsin was mixed preparation with TLCK at the concentration of 25 mg/mL and EDTA at the concentration of 1 mmol/L. Conclusion This study has obtained the better enzyme deactivation process which could be used for the detection of fibrinolytic activity of pepsin and trypsin degradation product by fiber fibrin plate assay; the operation is simple, and the repeatability and stability are good.
Objective To optimize the preparation of Saussureae Involucratae Herba extract (SIHE)-composite phospholipid liposome (CPL) by the central composite design-response surface methodology (CCD-RSM) and to investigate the in vitro release of drugs. Methods The method of ammonium sulfate transmembrane gradients was adopted to prepare SIHE-CPL. The single factor experiments were used for the key experimental factors and their test ranges. Based on the single factor experiments, with the size of SIHE-CPL, polymey disperse index (PDI), encapsulation efficiency (EE), and Zeta potential as dependent variables, central composite design was adopted to optimize the preparation technology by taking the content of phospholipid and the content of cholesterol as independent variables; test results were fitted by multiple linear and binomial nonlinear equations, and optimum formulation was selected by RSM; then the in vitro release behavior of the drug was studied with method of dynamic dialysis. Infrared (IR) spectroscopy and X-ray diffraction (XRD) pattern were used to analyze the spectroscopic properties of SIHE-CPL. Results The second-order polynomial equation was superior to the linear one, and the observed values agreed well with model predicted values. The optimal process conditions were as follows: size of SIHE-CPL was (102.7 ± 5.1) nm, PDI was 0.154 ± 0.017, EE of chlorogenic acid and rutin was (87.68 ± 2.57)% and (84.18 ± 2.97)%, Zeta potential was (−28.4 ± 2.2) mV, SIHE-CPL and solution of SIHE were both accorded with the first order model, and IR analysis and XRD proved the formation of SIHE-CPL. Conclusion The size and PDI of SIHE-CPL are low and the EE and Zeta potential of SIHE-CPL are high. CCD-RSM could be successfully used to optimize the prescription of SIHE-CPL.
Objective To prepare Rehmanniae Radix oligosaccharide micro-powders with high load capacity, high yield, and good moisture absorption, using nano spray drying technology. Methods Based on the single factor experiments, the B-90 spray dryer was used to prepare Rehmanniae Radix oligosaccharide micro-powders with average particle size, yield, and moisture absorption rate as evaluation indices, and orthogonal test was used to investigate the influence of inlet temperature, spray drying efficiency, and solution concentration on the preparation process and to optimize the preparation process. Results The best spray drying condition was A2B2C3, namely the inlet temperature was 110 °C, the spray drying efficiency was 50%, and the drug concentration was 1%. Finally the drug loading was 30% and the yield was 89% with good moisture absorption. The Rehmanniae Radix oligosaccharide micro-powders had good morphology stability. Conclusion The nano spray drying technology is stable and the micro-powders have good quality, which is better than the traditional spray drying technology.
Objective To analyze the change regulation of chemical constituent groups in processed aconite during decocting, and also to discuss the scientificity connotation of decoction of processed aconite. Methods An ultra-high performance liquid chromatography coupled with time of flight mass spectrometry (UHPLC-Q-TOF/MS) was carried out to acquire the chemical constituents information in samples with different decoction time. High precise mass data were processed by multivariate statistical analysis techniques to discover and identify the constituents with significant difference. Furthermore, the content change, toxicity, and activity of these constituents were also discussed. Results Fifteen chemical markers with significant difference were screened by partial least squares discriminant analysis (PLS-DA) and t test. By analyzing the change regulation of the different constituents during 4 h decocting process, we found that it mainly happened in the constituent dissolution and chemical transformation from monoester alkaloids to aconine alkaloids, which indicates the attenuation process is secondary. Conclusion In terms of analgesic and anti-inflammatory effect, it suggests that 0.5 h of decoction would be fine, while in terms of cardiotonic effect, it needs the further systemic comparison for cardiac activity between monoester alkaloids and aconine alkaloids to be able to investigate the necessary and scientificity for long time decoction of the processed aconite.
Objective To compare the relationship of ingredients and pharmacokinetics of steamed Notoginseng Radix et Rhizoma (the roots of Panax notoginseng) with different time (0, 2, 4, and 8 h), and accompany with its anti-platelet aggregation activity. Methods The components with different steaming duration were determined by HPLC method. Concentration of saponins in Notoginseng Radix et Rhizoma before and after steaming at different time points were detected by UPLC-MS/MS. Pharmacokinetic parameters of each compound were calculated using DAS 3.2.6 software. The anti-platelet aggregation activity was measured by platelet aggregation/clotting analyzer. Results The results showed that the steaming process reduced the contents of certain bioactive substances (NG-R1, Rg1, Rd, Rb1, and Re) and produced some new components (Rh1, Rg3, Rk3, and Rh4). Ginsenoside Rg3, deglycosylated metabolites of ginsenoside Rb1 possessed lower tmax than ginsenoside Rb1 that indicated the course of deglycosylation made faster absorption. Steamed Notoginseng Radix et Rhizoma had stronger antiplatelet activity, following higher antiplatelet and anticoagulant activities with increasing steaming durations. Conclusion The results inspire us that saponins may become more active ingredients after deglycosylation, saponins with deglycosylated in vitro become more and more active ingredients into the blood, which could make stronger anticoagulant activity.
