Supervisor(s):Chinese Pharmaceutical Association Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association ISSN:0253-2670 CN:12-1108/R
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica.
The journal is included in CA, JST and CSCD.
Objective To investigate the anti-atherogenic effects and possible mechanisms of the total saponins from Hemsleya chinensis (TSHC) on New Zealand rabbits with atherosclerosis (AS). Methods Thirty-six male New Zealand rabbits were randomly divided into six groups, namely control, model, TSHC 200, 100, 50 mg/kg, and simvastatin 200 mg/kg groups. In addition to control group fed with normal diet, the rest rabbits of other groups were given fat diet (79.5% basic feed + 15% yolk powder + 5% lard + 0.5% cholesterol) for three weeks, then removed cholesterol, continued to raise for three weeks, starting from modeling gavage once daily for six weeks. The serum triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), and low density lipoprotein-cholesterol (LDL-C) were detected in the third week. After administration of the sixth week, serum was analyzed for levels of TG, TC, HDL-C, LDL-C, apolipoprotein AI (Apo AI), apolipoprotein B (Apo B), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), nitric oxide (NO), endothelin (ET), thromboxane B 2 (TXB 2), tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8 (IL-8), 6-ketone prostaglandin F 1α (6-keto-PGF 1α), and related parameters of blood rheology. Calculation of aorta lipid area percentage, and pathological changes were observed under light microscopy. Results Compared with the model group, high- and mid-dose TSHC could significantly reduce the contents of TC and TG in the third week; high-dose TSHC could significantly raise the levels of HDL-C, Apo AI, SOD, GSH-Px, NO, and 6-keto-PGF 1α, while lower TC, TG, LDL-C, Apo B, ET, TXB 2, TNF-α, CRP, IL-6, and IL-8, the above indicators in mid- and low-dose groups also have varying degrees of rise and fall. High-dose TSHC could significantly reduce the blood viscosity (high shear and low shear), erythrocyte aggregation index, and the percentage of aortic lipid area, significantly improve vascular intima wall lipid deposition and wall thickening and luminal stenosis degree. Conclusion TSHC could inhibit the development of AS in rabbits, which might be due to adjusting blood lipid levels, anti-inflammatory effects, protecting vascular endothelial cell function, and improving the blood rheology.
Objective To construct high-throughput screening cell model targeting TAS2R14 receptor and lay the foundation for the search of effective, novel natural anti-asthma drugs with low toxicity. Methods The pLVX-AcGFP1-N1-TAS2R14 lentivirus vector carrying green fluorescent protein (GFP) was constructed. The lentiviral vector was transfected into HEK HEK293T cells, collected high titer lentiviral concentration liquid and infected HEK293T cells, established cell model highly specific expressed TAS2R14 receptor gene. The TAS2R14 cell model was used to screen 120 kinds of Chinese herb extracts and chemical monomers. Results The calculated Z′ values of the cell model were 0.69 and 0.66, and Citri Reticulatae Pericarpiumextract and its efficacy material limonin, Ginkgo Semen extract and its efficacy material rutin and quinine agitated TAS2R14 cell model. Conclusion The constructed TAS2R14 cell model is stable and sensitive for screening anti-asthma drugs, and three kinds of Chinese materia medica monomers have the potential agonist activity on TAS2R14 receptor.
Objective To study the effects of curcumin (CUR) on gut microbiota of interval sleep deprivation (ISD) rats. Methods Wistar rats were randomly divided into control group, big platform (BP) control group, model group, and CUR (70 mg/kg) group. Firstly, ISD model rats were established by improved small platform method; Then, open field test and sucrose preference test were utilized to assess the depression-like behavior of the ISD model rats and intervention effects of CUR; Thirdly, gut microbiota DNA samples were extracted from feces, the primers of Escherichia coli, Bifidobacterium, Lactobacillus, Clostridium perfringens, and Bacteroides were designed by 16 S rRNA gene sequence, and the fluprescence qualitation of the bacteria through general PCR was obtained. Results The results of behavioral experiments showed that open field test scores and sucrose preference rate were significantly lower than those of the BP group (P < 0.01), and CUR played a significant improving role. The qRT-PCR data suggested that the relative expression of E. coli, Bifidobacterium, Lactobacillus, and Bacteroides of ISD model rats were lower than those in normal and big platform groups in ISD model rats, while higher for Clostridium perfringens (P < 0.05, 0.01). The amounts of E. coli, Bifidobacterium, Lactobacillus, and Bacteroides of CUR intervened group were higher than those in ISD model rats, except for Clostridium perfringens (P < 0.05, 0.01). Conclusion The results show that as a stress, ISD not only causes mental disorders in rats, but also leads to the changes of five bacteria strains. CUR could alter the imbalance of gut microbiota, which might be one of the mechanisms of its effects on depression-like behaviors of ISD rats.
Objective To develop an HPLC-UV-ELSD method for the simultaneous determination of crenelatin, gallic acid, salidroside, tyrosol, harpagide, harpagoside, angoroside C, and cinnamic acid in Compound Rhodiola Capsule. Methods Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was adopted. The mobile phase was composed of acetonitrile (A) and 0.3% HAC (B) with gradient elution. The flow rate was 1.0 mL/min and the detection wavelength was 275 nm. the column temperature was 30 ℃, and the evaporative light-scattering detector (ELSD) drift tube temperature was 40 ℃, and gas pressure of 1.5 bar (150 k Pa). Results Crenelatin, gallic acid, salidroside, tyrosol, harpagide, harpagoside, angoroside C, and cinnamic acid were separated well. The linear calibration curves were obtained in 67.084–670.84 μg/mL for crenelatin, R2 = 0.999 2; 11.410–114.100 μg/mL for gallic acid, R2 = 0.999 4; 78.995–789.95 μg/mL for salidroside, R2 = 0.999 6; 19.625–196.25 μg/mL for tyrosol, R2 = 0.999 7; 59.368–593.68 μg/mL for harpagide, R2 = 0.999 8; 62.585–625.85 μg/mL for harpagoside, R2 = 0.999 5; 55.045–550.45 μg/mL for angoroside C, R2 = 0.999 6; and 6.895–68.95 μg/mL for cinnamic acid, R2 = 0.999 8. The average recoveries of the eight constituents were 100.8%, 98.9%, 100.1%, 100.8%, 98.9%, 99.6%, 100.7%, and 99.2% with RSD of 0.64%, 0.56%, 0.35%, 0.65%, 0.26%, 0.58%, 1.00%, and 0.64%. Conclusion The method is convenient, accurate, and can be used for the simultaneous determination of the preparation.
Objective To optimize the matrix proportion of SIS hot melt pressure sensitive adhesive suit for hydrophilic material releasing. Methods By orthogonal test method, taking adhesiveness and hydrophilic drug release rate as factors, and C5 petroleum resin hydrogenate, lanoline styrene-isoprene-styrene (SIS) triblock copolymer, ethyl acrylate-methyl methacrylate-trimethylamino (RLPO), liquid paraffin, dibutyl phthalate (DOP), polyethylene glycol (PEG) 400, and Antioxidant 1010 as materials, the matrix proportion was optimized. Results The optimized formulation was as follows: SIS-C5 petroleum resin hydrogenate-SIS-RLPO-DOP-PEG 400-Antioxidant 1010 was 3:4:6:6:5:1:0.1. The adhesiveness was proper and shows more release rate for hydrophilic material. Conclusion The SIS hot melt pressure sensitive adhesive shows good property and is suitable to prepare transdermal patch.
Objective To explore the mechanism of the compatibility of Mongolian medicine Sendeng-4. Methods Sendeng-4 was composed of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction with Gardeniae Fructus, Toosendan Fructus and Chebulae Fructus with a mass ratio of 5:3:1:1. BEH C18 column (50 mm × 2.1 mm, 1.7 μm) was used as the chromatographic column, water (0.1% formic acid)-methanol were used as mobile phase for the gradient elution, and the ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS), principle component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the chemical constituent changes of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction. Results The significant differences between the group of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and Sendeng-4 were observed in PCA and OPLS-DA model. There were significant differences among eight chemical compounds after compatibility (P < 0.05). The relative contents of catechin, (−)-gallocatechin, dihydromyricetin, quercetin decreased, xanthocerasic acid, 3β-hydroxytirucalla-7,24-dien-21-oic acid, 3-oxotirucalla-7,24-dien-21-oic acid, and rutin were increased. Conclusion Maybe the anti-inflammatory and anti-rheumatic activity of Sendeng-4 have the relationship with the content changes of some index component in Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium after compatibility.
Objective By HPLC, to explore the differences of adjuvants for the changes of chemical components of Rehmanniae Radix Praeparata during process by steamed for nine times and shined for nine times. Methods HPLC was conducted on a Hypersil GOLD aQ C18 column (250 mm × 4.6 mm, 5 μm), flow rate was 1 mL/min, and the column temperature was 25 °C. With the mobile phase of acetonitrilee and 0.1% phosphoric acid water (1:99), the detection wavelength was 210 nm (catalpol), 203 nm (rehmannioside A, rehmannioside D, and leonuride), acetonitrilee and 0.1% phosphoric acid water (3:97 and 4:96); 334 nm (actecosode), acetonitrilee and 0.1% phosphoric acid water (16:84), and 284 nm (5-HMF), acetonitrilee and 0.1% phosphoric acid water (11:89). Sugars was conducted on an Accurasil NH2 column (250 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrilee and 0.1% phosphoric acid water (72:28), flow rate was 1 mL/min, the column temperature was 25 °C. The detector was ELSD, Alltech2000ES, drift tube temperature was 93 °C, and the gas flow rate was 2.6 L/min. The statistic analysis was carried out by SPSS 20.0 and the paired sample t-test was carried out for 12 kinds of chemical composition in Rehmanniae Radix Praeparata obtained by steamed for nine times and shined for nine times and wine-steamed for nine times and shined for nine times. Results With the increase of steamed times, the contents of catalpol, leonuride, actecosode, stachyose, sucrose, and raffinose in Rehmanniae Radix Praeparata obtained by two ways are all decreased; while the contents of rehmannioside A and rehmannioside D were slightly increased; the contents of 5-HMF, fructose, anhydrous dextrose, and mannose trisaccharide were increased. Conclusion The established method conforms to the validation requirement of the methodology. In the process of Rehmanniae Radix Praeparata with steamed for nine times and shined for nine times, processing adjuvant has a marked impact on the quality of the processed product. And the related substances of Rehmanniae Radix Praeparata obtained by the two ways have a larger fluctuation in times 3, 4, and 6 of steamed and shined processes, and the reason for this still needs to be further studied.
