Supervisor(s):Chinese Pharmaceutical Association Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association ISSN:0253-2670 CN:12-1108/R
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica.
The journal is included in CA, JST and CSCD.
Objective: To multi-objectively optimize the purification process parameters of licorice flavonoids using entropy-weight method, Plackett-Burman design (PBD), and Box-Behnken design (BBD). Methods On the basis of HPLC fingerprints of licorice, the macroporous resin type was chosen using the recovery rates of six components (liquiritinapioside, liquiritigenin, isoliquiritinapioside, licuraside, isoliquiritin, and neoisoiiquiritin) as detection indexes. Weights of the recovery rates of six components were determined by entropy-weight method, in order to obtain the comprehensive index. The significantly influencing factors were firstly evaluated by PBD, then purification conditions were optimized by BBD. Results ADS-7 type resin showed a high selectivity for six components. The optimized purification technology was as follows: pH value was 4.5, sample concentration was 0.20 g/mL, sample volume was 1.0 g/g, flow rate was 0.6 mL/min, elution dosage was 6.7 BV, ethanol concentration was 83% of eluting agent, and elution rate was 1.0 mL/min. Under the conditions, the recovery rates of six components were 86%–97%, the theoretical and actual comprehensive indexes were 93.32% and 93.05%, respectively, with a relative error of 1.17%. Conclusion Entropy-weight method combined with PBD and BBD-RSM was used to optimize the purification process for the licorice flavonoids in this study is scientific and feasible, providing a new reference to realize the multi-objective optimization of the purification technology for active constituents in Chinese materia medica.
Objective A method of reversed phase high performance liquid chromatogram-light scattering detector (RP-HPLC-ELSD) was developed to separate and prepare a large amount of 25-OH-PPD epimeric mixture, in order to explore the best separation conditions between method of continuous sampling interval and off-line preparation method. Methods The separation consequences of the method of continuous sampling interval and off-line preparation method were investigated respectively, under different proportions of mobile phase and sample volumes, the optimum preparation method was screened by comparing the efficiency and transfer rate. Results The suitable operation conditions of the two methods and the result of the preparation were obtained: method of continuous sampling interval is as follow: the mobile phase is methanol and water (83:17), and the volume flow and injection volume are 20 mg/mL and 1 mL, respectively, while the flow rate is 20 mL/min; Under above conditions the preparation efficiencies of 20(S)-25-OH-PPD and 20(R)-25-OH-PPD are 18.01 and 35.36 mg/h, respectively; off-line preparation method, the mobile phase is methanol and water (81:19), and the volume flow and injection volume are 200 mg/mL and 2.5 mL, while the flow rate is 20 mL/min, The preparation efficiencies of 20(S)-25-OH-PPD and 20(R)-25-OH-PPD are 50.55 and 51.93 mg/h, respectively. Conclusion Preparation efficiency of off-line preparation method is higher than that of the method of continuous sampling interval. This method is convenient and reliable and has large amounts of 20(S)-25-OH-PPD and 20(R)-25-OH-PPD, which can establish a good foundation for the separation and preparation of 25-OH-PPD isomer.
Objective To explore the change of endogenous metabolites in serum of rats with erythronoclastic anemia and the intervention of Lvjiao Buxue Granules by using 1H-NMR metabonomics coupled with multivariate statistical analysis. Methods Acetylphenylhydrazine was used to establish the rat model of erythronoclastic anemia and after a week the rats except control and model groups were given to Lvjiao Buxue Granules(8 g/kg) separately once daily for two weeks. While the rats in the control and model groups were ig given equivalent distilled water respectively. The endogenous metabolites in serum from all of rats were analyzed by 1H-NMR coupled with multivariate statistical analysis. Results Compared with the control group, the levels of lipids, lactic acid, and acetone in serum of rats in the model group increased while the levels of alanine, valine, creatinine, phosphocholine, glycerophosphocholine, oxidation of three methyl ammonium, glycine, and arginine were decreased. The levels of these endogenous metabolites with significant difference were reversed to normal by ig asminstration of Lvjiao Buxue Granules. Conclusion The mechanism of Lvjiao Buxue Granules on tonifying blood is involved in the pathway of energy metabolomism, lipid metabolism, and intestinal bacteria metabolism.
Objective To explore the influence of extraction and concentration with long duration on the quality consistency of Qiongyu Paste (QYP), and to analyse the degradation and transformation mechanisms of each component involved in the quality change of QYP. Methods QYP was a paste formula derived from Rehmanniae Radix, Poria, and Ginseng Radix et Rhizoma in a weight ratio of 7:2:1, the contents of 10 major bioactive components [5-hydroxymethyl furfural (5-HMF), catalpol, melittoside, acetoside, ginsenoside Re, ginsenoside Rb1, ginsenoside 20(S)-Rg3, ginsenoside Rg1, ginsenoside Ro, and pachymic acid] were simultaneously determined by the previously established HPLC-MS method. The standard deviation (SD) accumulation values of the contents of 10 bioactive components in repeatedly prepared samples in different durations were compared. Results Total contents of the 10 components and relative contents of some individual components in QYP changed significantly with different extraction and concentration duration. At the same time, the SD values of the contents of bioactive components in repeatedly prepared samples decreased with extending the extraction and concentration duration. Conclusion Extraction and concentration could improve the quality consistency of QYP.
Objective The purpose of this paper is to verify the key role of water absorption in the process of disintegration of Chinese materia medica (CMM) dispersible tablets, so as to provide the solution idea for slow disintegration of CMM dispersible tablets. Methods In this paper, the influence of water absorption of raw materials and auxiliary materials on disintegration process was verified using oily Jianghuang Qingzhi Tablets (JQT) as research object. The water absorption and disintegrating process of different drugs, JQT with different materials, and modified dispersible tablet were determined. Results The water absorption of JQT was lower than that of lactose tablets, and the disintegrating process was slower. The water absorption of different materials prescription of JQT was: CMS-Na-PVPP (1:1) > PVPP > CMS-Na, and disintegrating process is the same as the former order. The water absorption of JQT after modification enhanced, and disintegration time was significantly shortened. It indicated that the water absorption of raw materials and excipients was stronger, the disintegration was faster. Conclusion The study proves that water absorption is a key role in the disintegration of CMM dispersible tablets. And co-grinding of silica powder and raw materials is important technology to solve the problem of slow disintegration of CMM dispersible tablets.
Objective To analyze the content of eight saccharides in unprocessed and processed Rehmannia glutinosa by HPLC and to investigate the impacts of processing time on saccharides in R. glutinosa. Methods HPLC conditions were as follows: Prevail Carbohydrate ES column (250 mm × 4.6 mm, 5 μm), flow rate of 0.8 m L/min, ELSD detector, acetonitrile as mobile A and water as mobile B for gradient elution. Results Fructose, glucose, sucrose, melibiose, raffinose, manninotriose, stachyose, and verbascose were separated with good linear relationships within their corresponding concentration ranges, the r values were within 0.998 4–0.999 7; the average recovery was 97.5%–103.2%. The content analysis at different processing time points showed that sucrose, raffinose, stachyose, and verbascose exhibited decreasing trend during processing, while fructose, glucose, melibiose, and manninotriose exhibited significantly increasing trend. Conclusion The proportions of saccharides in unprocessed and processed R. glutinosa are significantly different, and the results of this study could provide the data support for revealing the processing mechanism of R. glutinosa.
Objective To investigate the protecting and regulatory effects of water extract from Fructus Ligustri Lucidi (FLL) on bone structure and bone metabolism in osteoporosis rats. Methods SD female rats were bilaterally ovariectomized to establish osteoporosis model, and Sham operated rats only cut the fat around the ovary. Experimental rats were divided into four groups: Sham operation (SHAM) group, model (OVX) group, alendronic acid sodium (ALN) group, and Fructus Ligustri Lucidi (FLL) group, with nine rats in each group. The rats in FLL group were given FLL water extract (3.5 g/kg) and rats in ALL group were given ALN suspension (0.12g/kg) by ig administration for 12 weeks. At the end of the experiments, the contents of serum and urine calcium (S-Ca) and phosphorus (S-P), urine creatinine (U-Ca/Cr and U-P/Cr), serum high density lipoprotein (HDL-C) cholesterol, low density lipoprotein (LDL-C) cholesterol, total cholesterol (TC), and triglyceride (TG) were measured by biochemical methods. The levels of collagen I amino terminal peptide (PINP), collagen I carboxyl terminal peptide (CTX-I), osteocalcin (OCN), and urine deoxypyridinoline (DPD) were measured with ELISA. The determination of alkaline phosphatase (ALP) was by radioimmunoassay method. To evaluate the change of bone tissue structure, the bone density instrument, VivaCT, and a universal testing machine were used. Results FLL could inhibit the increased body weight of ovariectomy (OVX) rats, increase S-Ca, S-P, serum HDL and PINP contents, reduce urinary U-Ca/Cr and U-P/Cr ratios, reduce serum LDL-C, TC, TG, ALP, OCN, CTX, and reduce urinary DPD content (P < 0.05 or 0.01) in OVX rats. Meanwhile, FLL can elevate the femur head and vertebral bone mineral density, bone micro-structure and bone strength in OVX rats. Conclusion FLL can improve the bone density and bone strength in OVX rats by regulating Ca and P metabolism, collagen and non-collagen metabolism.
Objective To investigate the mechanism of flavonoids from Mosla scabra (FMS) on anti-influenza from the sight of microRNAs. Methods Mice were divided into normal group, model (MC) group, and FMS group. Mice in MC and FMS groups were infected with influenza virus H1N1, then mice in the FMS group were treated with FMS. To observe the influence of mice in FMS group for the lung index and the levels of cytokines in serum. The difference expressing of miRNAs in lung tissue of mice in each group were detected by high-flux sequencing and quantitative real-time PCR. Human mRNA database as target was used to predict the target genes of differentially expressed miRNAs by miranda, mirbase, and targetscan analysis, while the target genes functions were considered by KEGG analyses. The related proteins of target genes were tested by Western blotting. Results FMS could significantly decrease the lung index and cytokines of infected mice and regulate the expression levels of abnormal miRNAs tend to normal. We also found that miRNAs are relevant to JAK-STAT and TLR3 signal pathways by KEGG. Western blotting confirmed that FMS could adjust the abnormal protein level of infected mice. Conclusion FMS obviously alleviates viral pneumonia via regulating miRNA expression in mice.
