Supervisor(s):Chinese Pharmaceutical Association Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association ISSN:0253-2670 CN:12-1108/R
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica.
The journal is included in CA, JST and CSCD.
Objective To investigate the chemical constituents from Gleditsiae Spina (the thorns of Gleditsia sinensis). Methods The compounds were isolated and purified by silica gel, Sephadex LH-20 column chromatographic techniques, and their chemical structures were confirmed on the basis of spectroscopic analysis and the physicochemical properties. MTT colorimetry method was used to measure the inhibitory effect of the compounds on the proliferation of HepG2, A-549, and EC109 cells. Results Three coumarins were obtained from the ethyl acetate soluble fraction of the 90% ethanolic extract and their structures were identified as scoparone (1), isoscopoletin (2), and 6-amino-7-methoxycoumarin (3). Conclusion Compound 3 is a novel coumarin named gledisinmarin A. Compounds 1 and 2 are isolated from this plant for the first time. Compound 2 displays the stronger cytotoxicity against A549 cell with an IC50 value of 34.47 μg/mL, while cisplatin with an IC50 value of 11.50 μg/mL as a positive control.
Objective To establish an UFLC-MS method for simultaneous determination of eight active components (shikimic acid, citric acid, protocatechuate, chlorogenic acid, caffeic acid, cinnamic acid, oleanolic acid, and ursolic acid). Methods Shimadzu LC-20 A system was used, equipped with AB Sciex Qtrap 5500. The analysis was performed on a Poroshell 120 column (100 mm × 4.6 mm, 2.7 μm). The eight components were separated in 25 min with gradient mobile phase consisting of 0.5% CH3COOH-H2O (A) and 0.5% CH3COOH-CH3OH (B): 0–5 min, 10% A; 5–13 min, 10%–30% A; 13–15 min, 30%–95% A; 15–25 min, 95% A. The temperature of column was 30 ℃, and the injection volume was 2 μL. The multiple-reaction monitoring scanning (MRM) was employed for the quantification with switching electrospray ion source polarity in negative mode. The ion spray voltage was set at −4 500 V and the turbo spray temperature was maintained at 550 ℃. Results The eight components had a good linearity (r ≥ 0.999 2) within the linear ranges. The average recoveries were 96.14%–101.20%. The contents of the eight components in Chaenomelis Fructus varied from the different habitats. The contents of shikimic acid, citric acid, and chlorogenic acid were high while the content of caffeic acid was low. Conclusion The developed UFLC-MS method is simple, sensitive, and accurate and has the good repeatability in separation, which is available for the quality control of Chaenomelis Fructus.
Objective To study the secondary metabolites of Genkwa Flos(the buds of Daphne genkwa). Methods The compounds were separated and purified by silica gel chromatography and thin layer chromatography, and their structures were determined by analyses of the physicochemical properties and spectral data. Results Three lignans were obtained and identified as lariciresinol- 9-O-pentatriacontanoate(1), pinoresinol(2), 4-hydroxysesamin(3) from the ethanol extract of D. genkwa. Conclusion Compound 1 is a new tetrahydrofuranoid lignan, and known compound 3 is obtained for the first time from this plant.
Objective To optimize the acid hydrolysis process of burnet (Sanguisorbae Radix, the roots of Sanguisorba officinalis) total saponins and to improve the yield of the hydrolyzate burnet sapogenin. Methods Using orthogonal test design, the concentration of hydrochloric acid, solid-liquid ratio, hydrolysis temperature, and hydrolytic time for the hydrolysis process of burnet total saponins were investigated. Results The primary and secondary factors that influenced the hydrolysis were followed by order of the concentration of hydrochloric acid > hydrolytic time > solid-liquid ratio > hydrolytic temperature, preferably optimum for the solid-liquid ratio of 1: 200, hydrochloric acid concentration of 4 mol/L, hydrolytic time of 0.5 h, and hydrolytic temperature of 92 °C. Conclusion High yield of burnet sapogenin can be obtained by using the optimized hydrolytic conditions, which are suitable for industrial production.
Objective To optimize the best preparation technology for Jianchang Band processing adjuvant — honey rice chaff.Methods With the total flavonoids, ferulic acid, alcohol extracts, water extracts, appearance, and moisture as evaluation indexes,the L9(34) orthogonal design test was used to check the influence on the preparation technology of adjuvant-honey rice chaff by the amount of honey and water, frying time and temperature to determine the optimum preparation process. Results The best preparation of Jianchang Band honey rice chaff is determined as preparing with 30% of honey in rice chaff, 50% of water in honey,frying for 120 s at 90 °C. Conclusion The optimal preparation process is feasible.
Objective To research the chemical composition from the roots of Lonicera japonica and their anti-inflammatory activities. Methods The compounds were isolated and purified by chromatography on silica gel column, Sephadex LH-20 column preparative thin-layer, and semi-preparative HPLC, etc. Their structures were identified on the basis of spectroscopic data, and part of the isolated compounds were tested for their anti-inflammatory activities against zebrafish. Results Fourteen compounds were isolated and elucidated as 3,13-dihydroxystemodan-2-one (1), chrysophanol (2), palmarumycin CP2(3), β-sitosterol (4), stigmastero (5), stigmast-4,6,8 (14), 22-tetraen-3-one (6), erythrinassinate D (7), lanosterin (8), 3-O-acetyloleanolic acid (9), protocatechuin aldehyde (10), daucosterol (11), (E)-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)-2(3H)-furanone (12), lomacarinoside B (13), and (2E,6S)-8-(α-L-arabinopyranosyl-(1″→6′)-β-D-glucopyranosyloxy)-2,6-dimethyloct-2-eno-1,2″-lactone (14). Conclusion Compound 1 is a new compound,and compounds 2,3,6,7–9, and 13 are isolated from L. japonica for the first time. Compounds 9 and 10 show the significant anti-inflammatory activities at 100 μg/mL.
