Supervisor(s):Chinese Pharmaceutical Association Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association ISSN:0253-2670 CN:12-1108/R
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica.
The journal is included in CA, JST and CSCD.
Objective To establish a rapid and accurate method for the determination of 15 chemical drugs which were illegally added into the slimming Chinese patent medicines (CPM) and health foods. Methods The UPLC-MS/MS method was adopted. The samples were extracted with methanol by ultrasonic processing and separated on a Waters Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with 0.1% formic acid methanol (A)-0.1% formic acid water (B) as mobile phase by gradient elution (0–3 min, 33%–45% A; 3–5 min, 45%–55% A; 5–7 min, 55%–70% A; 7–9 min, 70%–80% A; 9–10 min, 80%–90% A; 10–11 min, 90%–33% A; 11–13 min, 33% A at a flow rate of 0. 2 mL/min, and the column temperature was 40 °C. A positive-ion (ESI+) source and an MRM mode were used to separate and quantitatively determine 15 chemical drugs. The obtained molecular ions, fragment ions, and retention time for MRM channels were used to identify the 15 kinds of drugs by comparison with those of reference substances. The obtained peak areas were used to determine the accurate contents of chemical drugs in CPM and the health foods. Results A good resolution of 15 kinds of chemical drugs, including terbutaline hydrochloride, ephedrine hydrochloride, theophylline, caffeine, doxofylline, clenbuterol hydrochloride, tulobuterol hydrochloride, bambuterol hydrochloride, fenfluramine hydrochloride, furosemide, indapamide, phenolphthalein, sibutramine hydrochloride, N-demethylated sibutramine hydrochloride, and hydrochloric acid N, N-dinor sibutraminel, was obtained under this UPLC and MS/MS condition. The limits of qualitation and quantitation were in the range of 0.1–5.0 ng/g and 0.3–15.0 ng/g. The standard addition recoveries were in the range of 91.8%–110.8%. In the 86 batches of samples (including capsule, granules, and other different matrix types) were detected in the 74 batches of added chemicals, the positive rate was 86.0%. Sibutramine hydrochloride (39 batches), furosemide (20 batches), phenolphthalein (23 batches), theophylline (1 batch), and caffeine (15 batches) were checked out in the samples, 22 batches of which two kinds were checked out, one batch of which three kinds were checked out. By contrast, the products which were not clearly marked manufacturer illegally added more seriously. Conclusion The method is simple, accurate, and highly sensitive, which can be used for the determination of illegally added chemical drugs in slimming CPM and health foods.
Objective To optimize a technology of integration on field processing and processing crude drugs of Polygoni Multiflori Radix (PMR) and to provide the scientific evidence for the integration of PMR. Methods Orthogonal test was used to optimize the integration technology on primary processing and reprocessing with two major characteristic components (stilbene glucoside and combined anthraquinone). To compare the differences between the integration technology and traditional technology on pharmacological activity by models of constipation and swelling of ear in mice with Various index (small intestinal propulsion rate, first defecation time, fecal water content, swelling degree of ear, and inflammatory factors). Results The results showed that the integration technology of 50 °C, 16 h was better, and there was significant difference in chemical composition and laxative effect between the integration technology and traditional technology. The anti-inflammatory effect of the integrated process was better than that of the traditional technology. Conclusion The technology of integration of field processing and processing crude drugs is feasible and the operation is good.
Objective To explore the mechanism of Evo (Evo) inducing cell cycle arrest and apoptosis in K562 cells. Methods The effect of Evo on proliferation of K562 cells was measured by Cell Counting Kit-8 assay (CCK-8 assay), and cell cycle distribution and apoptosis were determined by flow cytometry (FCM). Chemical colorimetry assay was used to examine the activity of histonemodification enzymes. The expression levels of histone deacetylase 6 (HDAC6), Cyclin D1, CDK4, Bcl-2, Bax, Cleaved Caspase-3, ERK, p-ERK, p38, and p-p38 proteins were ascertained by Western blotting. Results The proliferation of K562 cells was inhibited by Evo (1–16 μmol/L) in a dose- and time-dependent manner. FCM analyses revealed that Evo induced cell-cycle arrest in G0/G1 phase in K562 cells. The apoptosis rates of K562 cells were (11.47 ± 1.05) %, (12.77 ± 0.79) %, and (18.58 ± 1.37) % respectively after induced by Evo with different concentration (2, 4, and 8 μmol/L), which showed statistically significant difference compared with the control group (2.79 ± 1.01) % (P < 0.01). The activity of HDACs was reduced after treated with Evo (2, 4, and 8 μmol/L). Western blotting assay showed that the expression of Bax, Cleaved Caspase-3, p38, and p-p38 proteins increased, while CDK4, Cyclin D1,Bcl-2, HDAC6, ERK, and p-ERK proteins down-regulation after induced by Evo. Conclusion Evo can induce cell cycle arrest and apoptosis in K562 cells through the inhibition of HDAC6.
Objective To establish the HPLC fingerprint and analyze the correlation of Schisandrae Fructus (SF), intermediate, and finished products of Wuweizi Syrup. Methods The HPLC method was used with the condition that the column was ACE5-C18 (250 mm × 4.6 mm, 5 μm); The mobile phase was eluted with gradient by acetonitrile-water; The flow rate was 1.0 mL/min; The column temperature was 30 ℃; The detection wavelength was set at 218 nm. Results The content was determined and the fingerprint was established for SF from Changbai Mountain, intermediate, and finished products of Wuweizi Syrup; Eighteen common peaks of SF and 12 common peaks in intermediate and finished products of Wuweizi Syrup were marked. The chemical composition and content in ten batches of SF from Changbai Mountain were stability; The similarity from the same manufacturer, but different batches of the intermediate and finished product is greater; The correlation between medicinal materials and intermediates as well as finished products is better. Conclusion The established fingerprints have better reproducibility, which can be used for the quality control of Wuweizi syrup with good precision, accuracy, and reproducibility.
Objective To integrate UHPLC-Q Exactive Orbitrap-HRMS and HPLC-CL to study the major chemical constituents in Ziziphi Spinosae Folium(the leaves of Ziziphus jujuba var. spinosa) and to trace the active constituents on line. Methods A UHPLC-Q Exactive Orbitrap-HRMS method was developed for the identification of multi-constituents in Ziziphi Spinosae Folium. The fingerprint for the multi-component assay in the leaves was established by HPLC-UV. The biological activity fingerprint of Ziziphi Spinosae Folium scavenging H2O2 and O2·− was simultaneously identified for eight different areas in Shanxi. Results Ten compounds in the leaves were identified or tentatively characterized. Among them, six flavones have scavenging activities for free radicals. Thirteen common peaks were calibrated and the correlation coefficients were greater than 0.90. Conclusion A large number of flavones in Ziziphi Spinosae Folium have antioxidant activities. Ziziphi Spinosae Folium provides a novel, green, and sustainable source as antioxidant for the market.
Objective To investigate the chemical constituents of Dendrobium nobile from Guizhou province. Methods The constituents were isolated and purified by silica gel, MCI column chromatography, and preparative HPLC technology. The structure of the isolated compound was elucidated by MS and NMR spectra. Results A new sesquiterpene, identified as δ-cadinen-12,14-diol(1), was isolated from the stem of D. nobile. Conclusion Compound 1 is a new compound.
Objective To establish an optimization model for the extraction technology process of Honghua Tongluo Prescription (HTP) based on fuzzy analytic hierarchy process (FAHP). Methods The evaluation indexes tree was established which included objective level, criterion level, and index level. The criterion level consisted of effective constituent, impurity control, behavioristics, histopathology, and immune response. The index level consisted the extraction efficiency of hydroxysafflor yellow A (HSYA) and icariin, the content of total substances, mechano-allodynia, and mechano-hyperalgesia, the ratios of decentered nucleolus in dorsal root ganglion, the content of SP in pelma skin with plantar P quality as the index layer. Weighting coefficient of all the indexes was decided by FAHP based calculation. HTP extraction process route was comprehensively evaluated. Results The importance degrees (ω) of each criterion from high to low were: immune respons e(ω = 0.245), histopathology (ω = 0.23), effective constituent, behavioristics (ω = 0.20), and impurity control (ω = 0.125). The optimized extraction technology process was that Carthami Flos was better extracted by warm maceration with water at 60 °C, Epimedii Herba was circulated by reflux extraction with water. Cinnamomi Ramulus was better treated by reflux extraction with 80% alcohol. Conclusion An FAHP-based evaluation method with pharmacological indexes, effective constituent indexes, and impurity control as evaluation indexes, which could put formula Chinese materia medica principle and modern pharmaceutical engineering demands into consideration, is comprehensive and effective.
Objective Camptothecin (CPT) as a kind of poorly soluble drug was conducted micronization research. Methods CPT micronized powder was prepared by emulsification method using emulsification machine and high pressure homogenizer. Moreover, single factor method was utilized to optimize. Dissolution and oral bioavailability study on CPT micronized powder prepared under the best emulsification conditions was conducted. Results The best preparation conditions were as follows: the dosage of Tween-80 as the surfactant was 0.4%; the concentration of CPT in chloroform-methanol (8:2) mixed solution was 1 mg/mL; the volume ratio of water-organic phase was 7:3; the homogenization rate was 7 000 r/min for 11 min. The particle size of CPT micronized powder prepared under the optimized conditions was (165.6 ± 5.3) nm. The scanning electron microscopy (SEM) results showed that CPT micronized powder presented regular strip shape and uniform particle size. The dissolution study indicated that the solubility and dissolution rate of CPT micronized powder was increased by 1.36 and 4.09 times compared with the raw CPT powder. The results of oral bioavailability showed that compared with the raw CPT powder, the relative bioavailability of CPT micronized powder in rats was increased by 2.10 times. The results of gas chromatography showed that residual solvents in CPT micronized powder accorded with the standard of ICH. Conclusion Solubility and dissolution rate of CPT are both improved since CPT micronized powder is prepared by emulsification method. So the oral bioavailability is also improved.
Objective The lignan components in Schizandrae Fructus residue extracts were analyzed and evaluated. The protective effect of Schizandrae Fructus residue extracts on acute-hepatic damnification rat and the influence of that on intestinal flora were researched in the process of Shengmai Injection production, in order to provide the scientific basis for the development and utilization of Schizandrae Fructus extract. Methods Schizandrae Fructus residue extract was extracted by the 60% ethanol. The model of acute-hepatic damnification rats induced by CCl4 was prepared, the protective effects of Schizandrae Fructus residue extracts on hepatic-damnification rats were evaluated through the data of ALT, AST, ALB, and TP; The function of the extracts were evaluated by histopathological observation of the liver and colon of rats; Effect of extracts on hepatic-damnification rat’s intestinal flora was evaluated by the number and the number variation of the intestinal flora. Results The results showed that the mass fractions of schisandrin A, schizandrin B, schizandrin C, gomisin A, gomisin B, and schisantherin A were 1.44, 3.42, 1.68, 4.22, 2.92, and 0.58 mg/g, respectively. Pharmacodynamic evaluation showed that Schizandrae Fructus extracts can significantly reduce the content of AST and ALT (P < 0.01) and increase the content of ALB and TP in a certain extent in the serum of acute-hepatic damnification rats (P < 0.05); Mucous damage score of liver and colon is significantly decreased; Compared to liver injury group, Schizandrae Fructus extracts of high dose group and low dose group can significantly promote the proliferation of Lactobacillus and Bifidobacterium (P < 0.05) but reduce the number of Enterococcus and Enterobacteria in the intestinal tract of rats (P < 0.05). Conclusion Schizandrae Fructus residue extracts have the protective effects on the acute-hepatic damnification rats induced by CCl4, the effect of regulating intestinal flora and the proliferation of probiotics, the effect of protecting the constitution integrity of colon tissue, which provide a main basis for its further development and utilization.
