Supervisor(s):Chinese Pharmaceutical Association Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association ISSN:0253-2670 CN:12-1108/R
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica.
The journal is included in CA, JST and CSCD.
Objective To optimize the prescription of total alkaloid of Nelumbinis Plumula solid dispersion osmotic pump controlled release tablet and research its release characteristics in vitro. Methods The cumulative release percents in 2, 6, and 12 h and the linear correlation coefficient of cumulative release curve were taken as evaluation indexes to select the optimal prescription by using the central composite design response surface methodology (CCD-RSM). The main factors of influence on drug release, which were the dosage of NaCl and PEG400, and coating agent thickness. Results The optimal prescription for total alkaloid of Nelumbinis Plumula solid dispersion osmotic pump controlled release tablet were as follows: NaCl dose was 166.0 mg, PEG 400 content was 80.5%, and the coating weight gain was 3.5%. Conclusion The prescription optimization model of total alkaloid of Nelumbinis Plumula solid dispersion osmotic pump controlled release tablet is optimized by CCD-RSM, and it is proved to follow the zero-order release kinetics.
Objective To investigate the characteristics of multiple linear regression method, stepwise regression method, and standardized partial regression coefficient method for identification of critical process parameters. Methods The acid precipitation and alkaline dissolution process of Scutellariae Radix (SR) water extract was investigated as an example. Results Similar results were obtained using three different methods. The concentration of SR water extract, pH value of acid precipitation, pH value of alkaline dissolution, and refrigeration temperature were considered as critical process parameters. Conclusion The selection of threshold values of the three methods all is subjective. Multiple linear regression method is simple, but is not as sensitive as stepwise regression method. The effects of a parameter on several process indices can be considered simultaneously and easily weighted using standardized partial regression coefficient method.
Objective To explore the bidirectional solid fermentation transformation of 25 different edible fungal strains with Isatidis Radix (IR) and Isatidis Folium (IF) to get the synergism antibacterial effect of fermentation substance. Methods The dominant strains grown on IR and IF were obtained through initial screening and repeated screening. The antibacterial effect of fermentation product substance were determined by antibacterial test and TLC. Results The results showed that repeated screening got three strains which could grow fast in IR (G-11, G-13, and G-7) and IF (G-2, G11, and G-3). The G-3 strain and G-11 strain fermentation substance had better antibacterial effect than IR and IF respectively. The methanol extract of G-2 fermented IF substance appeared a new spot by TCL, it should be an additional component after fermentation. Conclusion The bidirectional solid fermentation could improved the antibacterial effect of IR and IF, and the new active ingredients could be produced.
Chinese medicine resources are the basis of the sustainable development of the health of Chinese medicine, and also the material basis for the sustainable development of Chinese medicine industry. Geoherbs are produced in the specific natural conditions, the ecological environment in the area of medicine. The quality of the medicinal materials is closely related to the characteristics of regional, diversity, variability, and humanity. It determines the particularity and regularity of the development of Chinese medicine resources. The effectiveness and safety of Chinese medicine are the core problems of the quality of Chinese medicine. However, the current Chinese medicine quality issues, quality evaluation, and control methods still exist and cannot objectively reflect the Chinese medicine problems in quality, quality standard in the determination and identification of components is not related with the effectiveness and safety. According to the relevance of the chemical constituents in Chinese medicines and plant secondary metabolite biosynthesis for reaction in herbs, herbal extracts, or compound preparation, and effects on the quality of medicinal materials, the author reiterated markers for defining the quality of traditional Chinese medicine basic conditions. Focusing on the material foundation of characteristic, difference, and dynamic change in whole process and quality transfer and traceability, the author believes that to establish quality markers of drug industry chain whole process control system, is conducive to the establishment of a full Chinese medicine quality control and quality traceability system.
Objective To prepare pH-dependent Paridis Rhizoma saponin (PRS) and Astragali Radix polysaccharide (ARP) colon targeting pellets for the treatment of colon cancer and finish its in vitro release performance evaluation. Method The colon targeted pellets were prepared with extrusion-spheronization and air-flow coating method and the the process parameters were optimized by orthogonal design. The coating fluid prescription was investigated by single factor test. In vitro release performance evaluation of the pellets was evaluated with polyphyllin I and II as the indexes. Result The optimum technologic parameters of extrusion spheronization equipment were as follows: the rate of extrusion was 60 r/min, the rate of spheronization was 1 200 r/min, and the time of spheronization was 5 min. The optimum coating fluid formulation of pH-dependent colon targetting pellets was 15% weight gains of Eudrugit S100, 1.5% anti-plastering aid amount of Glycerin monostearate and 5% plasticizer amount of TEC. In vitro release test showed that cumulative release rate of berberine hydrochloride was close to 0% in artificial gastric juice after 2 h and less than 10% in artificial intestinal fluid after 4 h, but the cumulative release rate in artificial colon juice after 2 h was more than 90%. Conclusion The preparation method can be applied to the preparation of colon targeted pellets and the pellets can achieve the targeted release in the colon.
Objective To investigate the anticancer effect and the mechanism of ginsenoside Rh2 on animal model of HepG2 liver carcinoma. Methods HE staining was used to observe cell morphology. Immunohistochemical staining was used to detect the expression of GSK-3β, β-catenin, and MMP-3 in isolated single cells. The activity of GSK-3β was checked by ELISA kit. The expression levels of GSK-3β, β-catenin, Bax, Bcl-2, Cyclin D1, and MMP-3 genes were measured by qRT-PCR. The expression of β-catenin and GSK-3β proteins were determined by Western blotting. Results HE staining showed that the nucleus was atypia and account for a large proportion of the whole cell in HepG2 group and HepG2-β-catenin group. But nucleus atypia in HepG2-β-catenin group was more obvious. Condensation nuclei and a lot of broken cells were observed in HepG2-β-catenin + ginsenoside Rh2 group and HepG2 + ginsenoside Rh2 group. However, condensation nuclei and broken cells in HepG2 + ginsenoside Rh2 group were more obvious. Immunohistochemical results indicated the expression of GSK-3β increased, while β-catenin and MMP-3 expression decreased in HepG2-β-catenin + ginsenoside Rh2 group, compared with HepG2-β-catenin group. The expression of β-catenin and MMP-3 in HepG2 + ginsenoside Rh2 group was lower than that in HepG2-β-catenin + ginsenoside Rh2 group, while GSK-3β was no significant difference. The ELISA results indicated that the activity of GSK-3β was increased in HepG2 + ginsenoside Rh2 group and HepG2-β-catenin + ginsenoside Rh2 group. Compared with HepG2-β-catenin + ginsenoside Rh2 group, the expression of Bax gene in HepG2 + ginsenoside Rh2 group increased significantly, and the expression levels of Bcl-2, CycliD1, and MMP-3 genes were also significantly lower; the difference was statistically significant (P < 0.01). The Western blotting results showed that compared with HepG2-β-catenin + ginsenoside Rh2 group, the expression of β-catenin protein in HepG2 + ginsenoside Rh2 group was also significantly lower, and the difference was statistically significant (P < 0.01). Conclusion In vivo experiment shows that weight of tumor is decreased by ginsenoside Rh2 through activating GSK-3β to degrade β-catenin and could inhibit the ability of HepG2 cells metastasis.
