Supervisor(s):Chinese Pharmaceutical Association Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association ISSN:0253-2670 CN:12-1108/R
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica.
The journal is included in CA, JST and CSCD.
Objective To optimize the extraction processes used for enzymatic hydrolysis of total sugar from Cynomorii Herba (Cynomorium songaricum) by employed quadratic orthogonal rotation regression combination design. Methods Taking the extraction rate of total sugar as index, as well as enzyme ratio, enzyme concentration, extraction temperature, extraction time, and initial pH value as investigation factors to screen out main factors. The quadratic orthogonal rotation regression combination design was applied to optimize the process. And then, to employ response surface analysis method (RSM) for evaluating the impacts on the response values of the single factor effect and interaction effect of each factor level. Results The optimum conditions were as follows: enzyme concentration was 1% (wt% of Cynomorii Herba powder), initial enzyme solution pH was 5.5, extraction temperature was 55 °C, extraction time was 45 min, and pectinase and cellulose was 1:1 proportion. The extraction yield of total sugar was (17.54 ± 0.35)%.The influence of response values of each factor presented the relationship of the parabolic surface with the opening downward. The results showed that the impact degree on total sugar yield of Cynomorii Herba were extraction temperature, extraction time, and enzyme ratio, respectively. Conclusion This method is a simple and effective method to increase the extraction yield of total sugar from Cynomorii Herba. Quadratic orthogonal rotation regression combination design has a favourable predictability.
Objective To study the extraction process of total triterpenoids of Antrodia camphorata (ACTT) and its anti-tumor activity. Methods Central composite design and response surface methodology (CCD-RSM) were applied to optimize the extraction process of ACTT. MTT assay was applied to detect the inhibitory effect of ACTT extract (ACTTE) on in vitro proliferation of cultured HepG2 cells. The morphological observation of HepG2 cells after being treated with ACTTE at the optimal mass concentration was performed under the luorescence microscope. The Annexin V apoptosis detection kit was used for detection of the apoptosis rate by flow cytometry. Results The optimized process was to perform the extraction at the ultrasonic power of 400 W with 20-fold the volume of 90% ethanol for two times, each lasting for 40 min. The extraction rate of ACTT was 11.87%, withdeviation between the actual and predicted values of 1.56%. The MTT assay showed that the ACTTE at different concentrations (12.5–200 μg/m L) had obvious inhibitory effects on the proliferation of Hep G2 cells (P < 0.01). The typical morphologic changes of apoptosis including pyknotic and fragmented nucleus, as well as apoptotic body could be observed under the microscope after Hoechst 33342 staining. The results of flow cytometry showed that the apoptosis rate in the drug administration group was significantly different from that in the control group (P < 0.01). Conclusion The ultrasonic extraction process of Antrodia camphorata with alcohol optimized by CCD-RSM is feasible due to the high goodness of fit between the actual and predicted values and good predictability. ACTT can significantly inhibit the in vitro proliferation of HepG2 cells and induce apoptosis.
Objective To establish rapid quantitative analysis models of multiple indicators for quality control in the concentrating process of Lvjiao Buxue Granule (LBG) by means of near infrared spectroscopy (NIRS). Methods The solid content (SC), total polysaccharides concentration (TPC), and ferulic acid concentration (FAC) were determined by weighing method, phenol–sulfuric acid method, and HPLC method, respectively. Calibration models of them were established with partial least squares regression (PLSR) method. The established models were applied to predicting the unknown samples for testing the performance of the models. Results The correlation coefficients (r) of SC, TPC, and FAC were all above 0.9450. The root mean square error in calibration (RMSEC) and root mean square error in prediction (RMSEP) were very close, and the relative standard errors of predictions (RSEP) were all less than 10%, indicating that the models predicted and performed well. Conclusion NIRS technique combined with chemo metrics can provide a novel efficient and environmental approach for fast simultaneous determination of key quality indicators friendly, and have potential application value in the concentrating process of LBG.
Objective: To explore the relationship between chemical components of Isatidis Radix (IR) and the inhibition of bioactivity of neuraminidase (NA) on influenza virus in vitro. Methods: To establish the chemical fingerprints of IR extracts from different producing regions and to determine the contents of common peaks by UPLC. To determine the activity of NA on influenza virus tested with IR by assay kits. To analyze the correlation of the chemical information and biological effects by statistical methods including grey correlation analysis and single-factor analysis. Results: The chemical components and biological effect detection varied markedly in the products from different regions and by different extracting methods. The result of comprehensive analysis showed that uridine and (R,S)-goitrin of common components in UPLC-fingerprints related with the inhibition of bioactivity of NA on influenza virus, and the content of (R,S)-goitrin was correlated significantly to the activity of NA. Conclusion: Based on the biological assays of NA on influenza virus, the correlation between the content of chemical component and biological effect is verified preliminarily, with a prospect to provide more reliable data and reference for selection and extraction of antiviral active ingredients from IR.
Objective To evaluate the quality of Citri Reticulatae Pericarpium Viride (CRPV) through the clinically-utilized hesperidin content. Methods On the basis of single-factor experiments, CRPV extraction technology was optimized with solvent volume and extraction time as independent variables, and the overall desirability value of extract yield and the extraction transfer rate of hesperidin as dependent variables. Central composite design was adopted to design a two factors-five levels table for quadratic-multinomial fitting. The extraction process was optimized with response surface methodology. According to the optimum extraction process, the clinically-utilized quantity of 11 batches of CRPV was determined based on the real content and extraction transfer rate. Results The optimal extraction technology is as follows. It was extracted for three times with 24 folds the amount of water, 2.5 h each time. The model-predicted and experimentally-measured values of overall desirability were 0.822 0 and 0.808 8, respectively, revealing the relative error of only 1.61% between them. The clinically-utilized quantity was not positively correlated to the contents of hesperidin of the 11 batches of CRPV. Conclusion The method established in this paper correlated the effective components of the medicinal materials to the clinical practice, which provided a new idea for the quality evaluation of Chinese medicinal materials.
Objective A high-content analysis (HCA) method was established for the in vitro evaluation of traditional Chinese medicine and the potential hepatotoxicity components and mechanism of Polygonum multijiorum were discussed. Methods HepG2 cells were incubated with different concentration and different incubation time of P. multijiorum extracts and components and then cells were stained with three fluorescent probes, then HCA was applied to detect the cell number, nucleus area, mitochondrial mass, and mitochondrial membrane potential. Results The ethyl acetate extract and dichloromethane extract had served hepatotoxicity. No significant changes were observed at low concentration (0.01 and 1 μmol/L) of P. multijiorum components; However, the cell number of aloe emodin, emodin, rhein, and gallic acid decreased significantly at the concentration of 0.1 μmol/L. Aloe emodin also caused the nuclear swollen. The TC50 values of emodin and rhein obtained from the dose-response curves were similar to the previous reports. Conclusion The aloe emodin, emodin, rhein, and gallic acid have potential hepatotoxicity that P. multijiorum induces liver injury. The hepatotoxicity of P. multijiorum may be related to mitochondria-mediated apoptosis according to the influence of P. multijiorum components on mitichondrial mass and mitochondrial membrane potential from the results. The HCA is applicable to evaluate the hepatotoxicity of traditional Chinese medicine.
