Establishment of chemiluminescent enzyme immunoassay for detecting antibodies against foot-and-mouth disease virus serotype O in swine
(2.University of Chinese Academy of Sciences, Beijing , China 100049)
(3.Department of Clinical Laboratory, First Hospital of Tsinghua University, Beijing , China 100016)
(4.China Institute of Veterinary Drug Control, Beijing , China 100081)
(5.Beijing Animal Health Inspection Institute, Beijing , China 100044)
【Abstract】Recombinant structural protein VP1 of foot-and-mouth disease virus serotype O was expressed in Escherichia coli and then purified using Nickel affinity chromatography. A chemiluminescent enzyme immunoassay (CLEIA) method was established using the purified recombinant protein as coating antigen to detect antibody of foot-and-mouth disease virus serotype O in swine. The specificity of VP1-CLEIA method is 100%. The coefficients of variation in the plate and between plates are 1.10%–6.70% and 0.66%–4.80%, respectively. Comparing with the commercial indirect ELISA kit or liquid phase block ELISA kit, the calculated coincidence rate is 93.50% or 94.00%. The high specificity and stability suggested this detection method can be used to monitor the antibody level of foot-and-mouth disease virus serotype O in swine.
【Keywords】 foot-and-mouth disease virus serotype O; VP1 protein; chemiluminescent enzyme immunoassay;
(Translated by ZHAO Bo)
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