Establishment of chemiluminescent enzyme immunoassay for detecting antibodies against foot-and-mouth disease virus serotype O in swine

Chen Cui1,2 Ligang Huang3 Jing Li1 Xingqi Zou4 Yuanyuan Zhu4 Lei Xie5 Qizu Zhao4 Limin Yang1 Wenjun Liu1

(1.CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing , China 100101)
(2.University of Chinese Academy of Sciences, Beijing , China 100049)
(3.Department of Clinical Laboratory, First Hospital of Tsinghua University, Beijing , China 100016)
(4.China Institute of Veterinary Drug Control, Beijing , China 100081)
(5.Beijing Animal Health Inspection Institute, Beijing , China 100044)

【Abstract】Recombinant structural protein VP1 of foot-and-mouth disease virus serotype O was expressed in Escherichia coli and then purified using Nickel affinity chromatography. A chemiluminescent enzyme immunoassay (CLEIA) method was established using the purified recombinant protein as coating antigen to detect antibody of foot-and-mouth disease virus serotype O in swine. The specificity of VP1-CLEIA method is 100%. The coefficients of variation in the plate and between plates are 1.10%–6.70% and 0.66%–4.80%, respectively. Comparing with the commercial indirect ELISA kit or liquid phase block ELISA kit, the calculated coincidence rate is 93.50% or 94.00%. The high specificity and stability suggested this detection method can be used to monitor the antibody level of foot-and-mouth disease virus serotype O in swine.

【Keywords】 foot-and-mouth disease virus serotype O; VP1 protein; chemiluminescent enzyme immunoassay;

【DOI】

【Funds】 Key Research Program of the Chinese Academy of Sciences (No. KSZD-EW-Z-005-001) Key Research Program of the Chinese Academy of Sciences(No.KSZD-EW-Z-005-001)

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(Translated by ZHAO Bo)

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This Article

ISSN:1000-3061

CN:11-1998/Q

Vol 32, No. 11, Pages 1519-1530

November 2016

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Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • References