Baicalein regulates Rho-ROCK-MLC pathway to inhibit the growth and metastasis of breast cancer
【Abstract】In this study, the inhibitory effect and mechanism of baicalein on tumor growth and lung metastasis of human breast cancer MDA-MB-231cells in nude mice was investigated. Twenty female BALB/C nude mice were randomized into normal group, model group, paclitaxel group, and baicalein group, with five mice in each group. The nude mice model of human breast cancer xenograft was established. Baicalein group was injected with 50 mg/kg baicalein, paclitaxel group 10 mg/kg paclitaxel, and normal group and model group normal saline for 8 weeks. Experimental results indicated that the tumor weight and the number lung metastatic nodules and metastases in paclitaxel group and baicalein group were smaller than those in the model group. Baicalein alone or in combination with ROCK inhibitor Y-27632 can inhibit the migration and cytoskeleton protein expression of MDA-MB-231 cells, reduce the formation of protein bundles, and inhibit the protein levels of ROCK1, Rac1, RhoA, and p-MLC.
【Keywords】 baicalein; breast cancer; cell migration;
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