Aronia melanocarpa anthocyanins protect SH-SY5Y cells against Aβ1-42-induced oxidative stress and apoptosis through a Nrf2 mechanism

MENG Ling-shuai1 CAO Sen1 PENG Lin1 MA Li-zhi1 MENG Xian-jun2 ZHOU Xiao-li1

(1.Food and Pharmaceutical Engineering Institute, Guiyang Universtiy, Guiyang 550005)
(2.Food Science College, Shenyang Agricultural University, Shenyang 110866)
【Novelty】It has been shown that anthocyanin is effective in reducing neuronal apoptosis by maintaining Ca2+ homeostasis, and can have a protective effect on neuronal cells. Previous studies have demonstrated the very high content and simple structure of anthocyanins in Aronia melanocarpa, consisting mainly of cornflower-3-O-galactoside, cornflower-3-O-glucoside, cornflower-3-O-arabinoside and cornflower-3-O xyloside, and the highly pure anthocyanins are relatively easier to isolate. Therefore, in this study, high purity anthocyanins from A. melanocarpa were selected to investigate the preventive and protective effects on oxidative stress-related Alzheimer's disease.

【Abstract】We investigated the cytoprotective effects of anthocyanins, including cyanidin-3-O-(galactoside, glucoside, arabinoside, xyloside), from Aronia melanocarpa against oxidative stress and apoptosis induced by Aβ1-42. The MTT assay was conducted by MTT colorimetric assay and cell group was done, oxidative stress related indicators, including reactive oxygen species (ROS), hydrogen peroxide (H2O2), superoxide dismutase (SOD), were measured using ROS, H2O2, SOD detection kit, respectively, apoptosis was detected by Annexin-V-PI/FITC apoptosis detection kit.Besides, the gene transcription and protein expression of antioxidase-related gene nuclear factor erythroid (Nrf2), heme oxygenase-1 (HO-1), quinone oxidoreductase (NQO1) and apoptosis-related genes (Bcl-2, Bax) were analyzed by RT-PCR and Western blot. These results showed that anthocyanins pretreatment significantly inhibited Aβ1-42-induced increase in intracellular ROS, H2O2, and increased the SOD expression through upregulating the Nrf2, HO-1, NQO-1 expression.Apoptosis was significantly inhibited by anthocyanins via down-regulation of Bax and up-regulation of Bcl-2. It can be concluded that high-purity A. melanocarpa anthocyanins could protect SH-SY5 Y cell from Aβ1-42-induced oxidative stress and apoptosis through a Nrf2 mechanism.

【Keywords】 Aronia melanocarpa; anthocyanins; SH-SY5Y cell; oxidative stress; apoptosis;

【DOI】

【Funds】 Project for Discipline Construction and Graduate Education of Guiyang University by Guiyang Finance (2021-XK15) Construction Project of Key Laboratory of Functional Food in Colleges and Universities of Guizhou Province (KY[2016]007)

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This Article

ISSN:1001-6880

CN:51-1335/Q

Vol 33, No. 07, Pages 1086-1095

July 2021

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Article Outline

Novelty

Abstract

  • 1 Materials and methods
  • 2 Result and analysis
  • 3 Discussion and conclusions
  • References