Establishment of screening method for salt-tolerant soybean at emergence stage and screening of tolerant germplasm

LIU Xie-Xiang1 CHANG Ru-Zhen1 GUAN Rong-Xia1 QIU Li-Juan1

(1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Germplasm Utilization, Ministry of Agriculture, Beijing, China 100081)
【Knowledge Link】linkage group

【Abstract】Salinity is an important factor affecting crop production. Screening salt-tolerant soybean germplasm is of great significance for the utilization of salinized soil in major soybean production regions. In order to select salt-tolerant soybean, a screening method was developed with six soybean accessions, including Zhonghuang 35, Zhonghuang 39, Williams 82, Tiefeng 8, Peking, and NY27–38. Seeds were sown in vermiculite and treated with 0, 100, and 150 mmol L−1 NaCl solution. Seedling rate (SR), plant height (H), fresh weight of shoot and root (FWS and FWR), dry weight of shoot and root (DWS and DWR) were decreased significantly under 150 mmol L−1 NaCl treatment, with significant difference among varieties. Therefore, 150 mmol L−1 NaCl was suitable to identify salt-tolerant soybean at emergence stage. The salt tolerance index (SI) based on the growth and development of seedlings and the salt tolerance coefficient (ST) were significantly correlated with the salt tolerance. The method using salt tolerance index is non-destructive and does not require planting control, which could save time and labor in salt-tolerant germplasm identification. Twenty-seven soybean resources were screened, in which three were highly tolerant (grade 1) and seven tolerant (grade 2) at emergence stage. Among them, Yundou 101, Zheng 1311, Wansu 1015, and Tiefeng 8 also showed salt tolerance (grade 1) at seedling stage. In summary, an effective method for screening salt-tolerant soybean at emergence stage was developed, with vermiculite as the substrate, 150 mmol L−1 NaCl as suitable treatment solution, and salt tolerance index as the indicator. Four soybean accessions were found to be salt tolerant at both emergence and seedling stages. This screening method will be useful for identification of salt-tolerant soybean germplasm.

【Keywords】 soybean; emergence stage; salt tolerance; screening method;

【DOI】

【Funds】 National Natural Science Foundation of China (31830066)

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(Translated by XU MY)

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This Article

ISSN:0496-3490

CN:11-1809/S

Vol 46, No. 01, Pages 1-8

January 2020

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Article Outline

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Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • 4 Conclusions
  • References