Overexpression of OsMPK17 protein enhances drought tolerance of rice

MA Jin-Jiao1 LAN Jin-Ping1,2 ZHANG Tong1 CHEN Yue1 GUO Ya-Lu1,3 LIU Yu-Qing1 YAN Gao-Wei1 WEI Jian1 DOU Shi-Juan1 YANG Ming1 LI Li-Yun1 LIU Guo-Zhen1

(1.College of Life Sciences, Hebei Agricultural University, Baoding, Hebei, China 071001)
(2.Research Center for Life Sciences, Hebei North University, Zhangjiakou, Hebei, China 075000)
(3.Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong, China 518116)
【Knowledge Link】overexpression; Western blotting

【Abstract】Mitogen-activated protein kinase (MAPK) highly conserved in eukaryotes plays important roles in stress responses of plant. In this study, the full-length OsMPK17 gene was cloned and fusion protein was expressed. The purified protein was used as immunogen to generate monoclonal antibody. Western blotting (WB) was carried out for protein samples isolated from tissues under different abiotic stresses. The expression of OsMPK17 was induced by drought stress, suggesting that the OsMPK17 protein may play a role in plant response to drought stress. Proteins isolated from leaves treated with abscisic acid (ABA) or methylene jasmonate acid (MeJA) demonstrated a decrease of OsMPK17 protein abundance, suggesting that hormones may be involved in the function of the protein. The overexpression vector of OsMPK17 protein was established and transformed into TP309 via Agrobacteria-mediated protocol. Homozygous transgenic lines for overexpression of OsMPK17 protein were obtained. In the field planting experiment, the transgenic lines showed shortened plant height, shortened spike length, and decreased seed setting rate. At seed germination stage, under the condition of PEG-6000 treatment, the seeds of OsMPK17-overexpressing lines grew better and the lengths of root and shoot were significantly longer than those of the wild type. The transgenic seedlings showed lower water loss rate when exposed in the air. The transgenic rice overexpressing OsMPK17 protein grew better than the wild type in the experiment with soil drought stress and re-watering then. In conclusion, the overexpression of OsMPK17 protein enhances drought tolerance of rice. This study enhances the understanding for the function of OsMPK17 protein.

【Keywords】 rice; MAPK protein; Western blot; stress; overexpression; antibody-based proteomics;

【DOI】

【Funds】 National Natural Science Foundation of China (31171528)

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This Article

ISSN:0496-3490

CN:11-1809/S

Vol 46, No. 01, Pages 20-30

January 2020

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Abstract

  • 1 Materials and methods
  • 2 Results
  • 3 Discussion
  • 4 Conclusions
  • References