Correlation between chemical composition in process of Isatidis Radix preparation and its pharmacological activity

HU Yan-jun1 WANG Ya-qi1 LI Bing-tao1 LAN Ji-ping1 WU Zhen-feng1 XIONG You1 YANG Ming1

(1.Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, China 330004)

【Abstract】Objective To investigate the key factors in the process of Isatidis Radix (IR) preparation. The content changes of its main chemical ingredients (epigoitrin, uridine, guanosine, adenosine, and indirubin) and the correlation with its pharmacological activity were studied, which would provide theoretical foundation for further optimization of process. Methods The process of IR preparation (extracting–concentrating–alcohol sinking–drying–granulation) was simulated. HPLC was employed to determine the contents of the chemical ingredients in each step of IR preparation, and the clearance rate of OH· was used as the model to investigate the antioxidant activity. Cluster analysis, partial least sqμares (PLS), and multiple linear regression (MLR) were used to analyze the correlation between its chromatogram and pharmacological activity. Results The content changes of chemical ingredients from IR preparation were generally decreased on the whole, and the highest loss rate of chemical ingredients occurred in the concentrating–drying process. Two metrological methods were used to analyze the correlation and the chromatogram of indirubin, guanosine, and adenosine, which was positively related with the clearance rate of OH·. Conclusion Each step in the process of IR preparation had the significant effect on the content of its chemical ingredients and pharmacological activity. The concentrating and drying process may be the key process which would finally influence the quality of preparation. Indirubin, guanosine, and adenosine may be the effective substances of IR with antioxidant activity.

【Keywords】 Isatidis Radix; process; chemical composition; pharmacological activity; correlation; epigoitrin; uridine; guanosine; adenosine; indirubin;

【DOI】

【Funds】 National Natural Science Foundation of China (Grant No. 81560649) Program for Key Discipline of Jiangxi University of Traditional Chinese Medicine (2013jzzdxk048) Science and Technology Project of Jiangxi Provincial Department of Education (GJJ14623)

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This Article

ISSN:0253-2670

CN:12-1108/R

Vol 47, No. 09, Pages 1515-1519

May 2016

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Article Outline

Abstract

  • 1 Instruments and materials
  • 2 Methods and results
  • 3 Discussion
  • References