Correlation between chemical composition in process of Isatidis Radix preparation and its pharmacological activity
【Abstract】Objective To investigate the key factors in the process of Isatidis Radix (IR) preparation. The content changes of its main chemical ingredients (epigoitrin, uridine, guanosine, adenosine, and indirubin) and the correlation with its pharmacological activity were studied, which would provide theoretical foundation for further optimization of process. Methods The process of IR preparation (extracting–concentrating–alcohol sinking–drying–granulation) was simulated. HPLC was employed to determine the contents of the chemical ingredients in each step of IR preparation, and the clearance rate of OH· was used as the model to investigate the antioxidant activity. Cluster analysis, partial least sqμares (PLS), and multiple linear regression (MLR) were used to analyze the correlation between its chromatogram and pharmacological activity. Results The content changes of chemical ingredients from IR preparation were generally decreased on the whole, and the highest loss rate of chemical ingredients occurred in the concentrating–drying process. Two metrological methods were used to analyze the correlation and the chromatogram of indirubin, guanosine, and adenosine, which was positively related with the clearance rate of OH·. Conclusion Each step in the process of IR preparation had the significant effect on the content of its chemical ingredients and pharmacological activity. The concentrating and drying process may be the key process which would finally influence the quality of preparation. Indirubin, guanosine, and adenosine may be the effective substances of IR with antioxidant activity.
【Keywords】 Isatidis Radix; process; chemical composition; pharmacological activity; correlation; epigoitrin; uridine; guanosine; adenosine; indirubin;
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References
[1] Chinese Pharmacopoeia [S]. Volume I. 2010 (in Chinese).
[2] Ma Y M, Li N, Liu C W, et al. Comparative studies on anti-inflammatory and analgesic activities of different fractions from Isatidis Radix [J]. Chinese Traditional and Herbal Drugs, 2014, 45 (17): 2517–2521 (in Chinese).
[3] Jin Y Z, Song G M, Zhao Y W, et al. Screening of active fractions of radix isatidis on immunologic function [J]. Acta Academiae Medicinae CPAF, 2011, 20 (7): 538–540 (in Chinese).
[4] Hu X Y, Liu M H, Sun Q, et al. Spectrum–effect relationship of antibacterial extracts from Isatidis Radix [J]. Chinese Traditional and Herbal Drugs, 2013, 44 (12): 1615–1620 (in Chinese).
[5] He Y Y, Zhao Z J, Zhao M. 板蓝根含片抑菌、抗炎作用的实验研究 [J]. Modern Traditional Chinese Medicine, 2006, 26 (2): 57–58 (in Chinese).
[6] Xiao S S, Jin Y, Sun Y Q. Recent progress in the studies of chemical constituents, pharmacological effects and quality control methods on the roots of Isatis indigotica [J]. Journal of Shenyang Pharmaceutical University, 2003, 20 (6): 456–458 (in Chinese).
[7] Dou X B, Yu X L, Wang Y. RP-HPLC法测定板蓝根中腺苷的含量 [J]. Qilu Pharmaceutical Affairs, 2004, 23 (7): 28–29 (in Chinese).
[8] Huang F, Xiong Y T, Yu L H. Pharmacokinetics of epigoitrin, an anti-virus component of Isatis indigotica, in different extraction in rats [J]. Journal of China Pharmaceutical University, 2006, 37 (6): 519–522 (in Chinese).
[9] Lmo Y, Zhang J, Zhao H P, et al. SimμLtaneoμs determination of chlorogenic acid, phillyrin, arctiin, liqμiritin and glycyrrhizin in yinqiao powder by HPLC [J]. Chin Pharm J, 2013, 48 (6): 473–476.
[10] Huang J D. 板蓝根化学成分和药理作用综述 [J]. Chinese Journal of Modern Drug Application, 2009, 3 (15): 197–198 (in Chinese).
[11] Lei L M, Pan Q P. Recent Progress in the Studies of Chemistry, Pharmacology, Quality Control and Extraction Methods on the Radix Isatidis [J]. Lishizhen medicine and materia medica research, 2007, 18 (10): 2578–2580 (in Chinese).
[12] Sun Q, Li H B, Yan D et al.. Evaluation on quality change of Isatidis Radix Granule during preparation process based on antibacterial potency [J]. Chinese Traditional and Herbal Drugs, 2012, 43 (2): 259–264 (in Chinese).
[13] Luo Y, Jin Cm Yan D. Effect of different drying methods on active components in water extracts from Isatidis Radix [J]. Chinese Traditional and Herbal Drugs, 2011, 42 (8): 1532–1536 (in Chinese).
[14] Tan M R, Yan D, Qiu L L, et al. Investigation on production process quality control of traditional Chinese medicine—Banlangen granule as an example [J]. China Journal of Chinese Meteria Medica, 2012, 37 (8): 1122–1126 (in Chinese).
ISSN:0253-2670
CN:12-1108/R
Vol 47, No. 09, Pages 1515-1519
May 2016
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