Optimization of technique for additional liquid fermentation of Poria cocos and comparison on chemical constituents from different sources of Poria
【Abstract】Objective To optimize the technique for additional liquid fermentation of Poria cocos in fermentor and to compare the chemical constituents in natural Poria, fermented Poria, medicinal fermented Poria, and compound medicinal fermented Poria. Methods The additional liquid fermentations of Poria cocos in fermentor had been carried out among different initial fermentation media, such as nutrition medium, medicinal medium, and compound medicinal medium. An optimum initial fermentation medium had been screened and an optimum time of additional liquid fermentation had been determined. The chemical constituents from the different sources of Poria had been isolated and detected according to their physicochemical properties. Results The compound medicinal medium with Coicis Semen and Lycii Fructus was an optimum initial fermentation medium, and 120 h was an optimum time of additional liquid fermentation. The total saccharides, polysaccharides, amino acids, trace elements, and ashes in the different sources of Poria had been detected. Conclusion There are considerable influences on liquid fermentation of Poria cocos in different initial fermentation media. There are rather big differences of chemical constituents from the different sources of Poria.
【Keywords】 Poria cocos; additional liquid fermentation; initial fermentation medium; chemical constituents; mycelium;
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