Migration study on ceramic membrane separation process of active ingredient population in compound Chinese materia medica

XU Yi-qing1,2 YANG Hui2 LUO You-hua2 LI Han-ming1,2 XU Guang-hui2 HUANG Yi-qi2

(1.College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China 350122)
(2.Xiamen Key Laboratory of Natural Medicine Research and Development, Xiamen Medicine Institute, Xiamen, China 361008)

【Abstract】Objective Taking the water extraction of Compound Banlangen Liyan Granules (Isatidis Radix, Scrophulariae Radix, Platycodi Radix, Glycyrrhizae Radix, etc.) as the research object to explore the dynamic migration regular of the active ingredient population of compound Chinese materia medica in different apertures in ceramic membrane separation process and compare its membrane filtration flux and solid inclusion removal rate. Methods The water extraction of Compound Banlangen Liyan Granules was separated with 200 nm and 50 nm aperture of ceramic membrane, respectively. Continuously sampling for many times was done in the ceramic membrane separation process. The optimized HPLC methods were as follows: chromatographic column was Agilent Zorbox Eclipse XDB-C18, mobile phase was acetonitrile-0.05% phosphoric acid in gradient elution at flow rate of 1 mL/min, detection wavelength was wavelength switching, injection volum was 10 μL, the column temperature was 30 ℃. The contents of six active ingredients [adenosine, (R,S)-goitrin, liquiritin, harpagosid, platycodin D, and monoammonium glycyrrhizinate] were determined in the compound at the same time, and the dynamic migration rates were investigated. Results The simultaneous determination of the six active ingredient in the compound was done with HPLC method. For the water extraction in Compound Banlangen Liyan Granules, in the 200 nm ceramic membrane separation process, the dynamic migration rates of the six active ingredients ranged between 71%–104%, the average migration rate was 85%, the membrane filter flux attenuation was smaller, the stable flux was in 426–340 L/(m2·h), solid inclusion removal rate was 21.0%. But in 50 nm ceramic membrane, the dynamic migration rates of the six active ingredients ranged between 83%–107%, the average migration rate was 83%, the membrane filter flux attenuation was larger, the stable flux was in 258–228 L/(m2·h), and solid inclusion removal rate was 23.9%. Conclusion The experiment preliminarily reflects the dynamic migration regular of the active ingredient population of compound Chinese materia medica in two different aperture ceramic membrane separation process. It lays a migration theoretical foundation of effective materials for popularization and application of modern ceramic membrane technology in Chinese materia medica refining.

【Keywords】 ceramic membrane separation; Compound Banlangen Liyan Granules; adenosine; (R,S)-goitrin; liquiritin; harpagosid; platycodin D; monoammonium glycyrrhizinate; migration;

【DOI】

【Funds】 Medical Innovation Project of Fujian Province, China (2012-CXB-40) Guiding Program for Science and Technology Program of Xiamen Municipal Bureau science and Technology, China (2011S0554)

Download this article

    References

    [1] Guo Liwei. Membrane Science and Technology, 2003, 23(4): 209-213 (in Chinese).

    [2] Li Cunyu, Ma Yun, Chen Tao, et al.Establishment of a rapid method to evaluate ultrafiltration membrane aperture for pharmaceutical industry. Chinese Traditional and Herbal Drugs, 2015, 46(11):1603-1608 (in Chinese).

    [3] Wu Lihua, Huang Ying, Liu Ting, et al.Drug Evaluation Research, 2014, 37(2): 184-187 (in Chinese).

    [4] Cen Qin, Zhou Lili, Li Tong. Journal of Shenyang Pharmaceutical University, 2008, 25(1): 77-80 (in Chinese).

    [5] Song Zhongxing, Tang Zhishu, Liu Hongbo, et al. Chinese Traditional and Herbal Drugs, 2015, 46(12): 1774-1778 (in Chinese).

    [6] Huang Minyan, Pan Linhai, Guo Liwei. Chinese Traditional Patent Medicine, 2010, 32(3):495-497 (in Chinese).

    [7] Luo Youhua, Yang Hui, Huang Yiqi, et al.China Journal of Experimental Traditional Medical Formulae, 2013, 19(1):287-290 (in Chinese).

    [8] Luo Youhua, Yang Hui, Huang Yiqi, et al.Strait Pharmaceutical Journal, 2013, 25(6): 18-21 (in Chinese).

    [9] Luo Youhua, Yang Hui, Huang Yiqi, et al. Simultaneous determination of six components in Compound Banlangen Liyan Granules by HPLC. Chinese Traditional Patent Medicine, 2014, 36(7): 1435-1439 (in Chinese).

    [10] Luo Youhua, Li Fucheng, Yang Hui, et al.Study on optimizing the extraction and refining technology of Yanshuning granules. Strait Pharmaceutical Journal, 2010, 22(11): 18-21 (in Chinese).

    [11] Luo Youhua, Li Fucheng, Yang Hui, et al. Study on Purifying Decoction of Yanshuning Compound by Ceramic Membrane Microfiltration Technology. China Journal of Experimental Traditional Medical Formulae,2010, 16(13): 12-16 (in Chinese).

    [12] Fang Jianguo, Tang Jie, Yang Zhanqiu, et al.Chinese Traditional and Herbal Drugs, 2005, 36(2): 242-244 (in Chinese).

    [13] Xu Lihua, Huang Fang, Chen Ting, et al.Chinese Journal of Natural Medicines, 2005, 3(6): 359-360 (in Chinese).

    [14] Chen Kai, Dou Yue, Chen Zhi, et al.China Journal of Experimental Traditional Medical Formulae, 2011, 17(18): 275-278 (in Chinese).

    [15] Hu Yingying, Huang Zhen. Advances in Research of Chemical Constituents and Pharmacological Effects of Scrophularia Ningpoensis Hemsl. Journal of Zhejiang University of Traditional Chinese Medicine, 2008, 32(2): 268-270 (in Chinese).

    [16] Xie Lihua, Liu Hongyu, Qian Ruiqin, et al.Journal of Peking University: Health Sciences, 2001, 33(3): 283-284 (in Chinese).

    [17] Zhang Jianchun, Zhu Jianmei. Shandong Medical Industry, 2003, 22(1): 25-27 (in Chinese).

    [18] Li Ting, Xu Wenshan, Li Xiwen, et al.Pharmacology and Clinics of Chinese Materia Medica, 2013, 29(2): 205-208 (in Chinese).

    [19] Gao Xueyan, Wang Wenquan, Wei Shengli, et al. Review of pharmacological effects of Glycyrrhiza Radix and its bioactive compounds. China Journal of Chinese Materia Medica, 2009, 34(21):2695-2700 (in Chinese).

This Article

ISSN:0253-2670

CN:12-1108/R

Vol 47, No. 09, Pages 1525-1530

May 2016

Downloads:2

Share
Article Outline

Abstract

  • 1 Instruments and materials
  • 2 Method and results
  • 3 Discussion
  • References