Effect of total saponins of Panax notoginseng on neuroregeneration in subventricle zone of rats with global cerebral ischemia

HE Xu1,2 GE Jin-wen3 HUANG Jun1,4 PAN Ai-hua1 LI Zhi-yuan1

(1.Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China 410013)
(2.Department of Anatomy, Yiyang Medical College, Yiyang, China 413000)
(3.Department of Integrated Traditional and Western Medicine, Hunan University of Traditional Chinese Medicine, Changsha, China 410208)
(4.Department of Anatomy, Shaoyang Medical College, Shaoyang, China 422000)

【Abstract】 Objective To explore the effect of total saponins of Panax notoginseng (TSPN) on the neuroregeneration in subventricle zone (SVZ) in rats with global cerebral ischemia. Methods Using four-vessel occlusion method to build the global cerebral ischemia model. Rats were divided into Sham group, model group and TSPN group. The rats in TSPN group were ip administered with TSPN 30 min post-brain ischemia. The dose of TSPN (75 mg/kg) was suspended in 0.9% saline (10 g/L), once per day for 1, 3, 7 and 14 d after reperfusion. While rats in the model group were treated with equal volume of 0.9% saline, one injection per day until the rats were sacrificed at either 1, 3, 7 and 14 d after brain ischemia. The BrdU and Doublecortin (DCX) expression in SVZ was assessed by immunohistochemistry and applying the immunofluorescence double-labeling to detect the BrdU/DCX, DCX/Ki67, and GFAP/DCX in SVZ. Results In comparison with the model group, the number of BrdU+ cells in SVZ of TSPN group was significantly higher on days 7 and 14 (P < 0.01, 0.001); statistical meaning existed in two groups on days 7 and 14 about the mean optical density of DCX+ cells in SVZ (P < 0.01, 0.001). In comparison with the model group, the number of the BrdU-labeled cells co-expressing DCX in the SVZ on day 14 of TSPN group was significantly different (P < 0.01, 0.001). There was statistical meaning in comparison of the number of colocalization of DCX with Ki67 on days 7 and 14 in SVZ between the TSPN group and model group (P < 0.01, 0.001). The ratio of GFAP/DCX to DCX in SVZ of two groups were statistically different on days 3, 7 and 14 (P < 0.05, 0.001). Conclusion TSPN could promote the neuroregeneration, drive the proliferation and differentiation of neural progenitor cells, and enhance the differentiation of gliosis into newborn immature neurons in SVZ of rats with global cerebral ischemia.

【Keywords】 total saponins of Panax notoginseng; SVZ; neuroregeneration; migration; global cerebral ischemia;

【DOI】

【Funds】 National Natural Science Foundation of China (81171037/H0903) Innovation Platform and Talent Program of Hunan Provincial Science & Technology Department (2015JC3129) Doctoral Innovative Research Topic of Hunan Province (CX2014B099) Project of Education Department of Hunan Province (13C958) Project of Science and Technology Bureau of Shaoyang City (2015JH51) Project of Science and Technology Bureau of Yiyang City (2015JZ42)

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This Article

ISSN:0253-2670

CN:12-1108/R

Vol 47, No. 09, Pages 1535-1540

May 2016

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Methods
  • 3 Results
  • 4 Discussion
  • References