Identification of Bupleuri Radix and its adulterants by DNA barcoding

HAN Xiao-wei1 YAN Yu-ping1 WU Lan-fang1 WANG Hong-fang1 SONG Jun-na1 WANG Qian1 ZHENG Yu-guang1

(1.Hebei University of Chinese Medicine, Shijiazhuang, China 050200)

【Abstract】Objective To identify the authenticity of Bupleuri Radix in the market of Hebei province. Methods In this research, the ITS2 sequence of 28 samples of Bupleuri Radix that were bought from the market in Hebei province was amplified by PCR, and the sequence of seven samples was downloaded from Gen Bank. Aligner Codon Code software was used for sequence alignment, the MEGA6. 0 analysis was used to calculate the K2P distance, and the mutation sites of each sequence was analyzed. Results Among the 28 Bupleuri Radix samples, there were 21 samples for Bupleurum chinese, 3 for Bupleurum smithii, and 4 for the false. Conclusion ITS2 sequence can be used as an effective method to identify the authenticity of BupleuriRadix, it is great value to the medicine market standard and the clinical medication safety.

【Keywords】 Bupleuri Radix; ITS2 sequence; Chinese medicinal materials; identification; barcoding;

【DOI】

【Funds】 Project of Administration of Traditional Chinese Medicine of Hebei Province (2015081) Doctoral Fund for Scientific Research by Hebei University of Traditional Chinese Medicine (BSZ2015003); Key Discipline of Hebei College of Traditional Chinese Medicine Research Fund for Youths by Hebei Provincial Education Department (QN2015073) Chinese Herbal Medicine Innovation Team Project of Modern Agricultural Technology System by Hebei Province (7000120081)

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(Translated by 易涛)

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This Article

ISSN:0253-2670

CN:12-1108/R

Vol 47, No. 09, Pages 1583-1588

May 2016

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Article Outline

Abstract

  • Materials
  • 2 Method
  • 3 Results
  • 4 Discussion
  • References