High-throughput screening of ochratoxin A in Chinese herbal medicines using enzyme-linked immunoassay

QIN Lu1,2 ZHANG Lei2 JIANG Jia-yi2 WANG Chang-jian2 DOU Xiao-wen2 WAN Li1 YANG Mei-hua2

(1.College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China 611137)
(2.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China 100193)

【Abstract】An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for the rapid detection of ochratoxin A (OTA) in nutmeg (Myristicae Semen), ginger (Zingiberis Rhizoma) and turmeric (Curcumae Longae Rhizoma). The matrix matching standard curve was used instead of the standard curve of sample diluent, and the sample extract and sample diluent were optimized. The sensitivity (IC50) of this method for OTA in nutmeg, ginger and turmeric were determined as 0.146, 0.157 and 0.153 ng·mL−1, respectively and the limits of detection (LODs) were 0.040, 0.032 and 0.031 ng·mL−1, respectively. The recoveries of samples ranged from 75.99% to 122.3%, with RSD < 10%. Two positive samples for nutmeg and one positive sample for turmeric occurred in 50 samples, and the highest OTA contamination value was 1 167.8 μg·kg−1. The results were further confirmed by LC-MS/MS. It shows that the developed ic-ELISA method is simple, rapid and sensitive, and can be applied for rapid and high-throughput screening of OTA in nutmeg, ginger and turmeric, as well as some other Chinese herbal medicines.

【Keywords】 indirect competitive enzyme-linked immunosorbent assay; Myristicae Semen; Zingiberis Rhizoma; Curcumae Longae Rhizoma; ochratoxin A; rapid screening;

【DOI】

【Funds】 National Natural Science Foundation of China (81872999, 81703699) Medical and Health Science and Technology Innovation Project of the Chinese Academy of Medical Sciences (2017-I2M-1-013) National Key Research and Development Program of China (2016YFE0112900) National Overseas Experts Program (G20190001631)

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This Article

ISSN:1001-5302

CN:11-2272/R

Vol 44, No. 23, Pages 5072-5077

December 2019

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Abstract

  • 1 Materials
  • 2 Methods
  • 3 Results and analysis
  • 4 Discussion and conclusion
  • References