High-throughput screening of ochratoxin A in Chinese herbal medicines using enzyme-linked immunoassay
(2.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China 100193)
【Abstract】An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for the rapid detection of ochratoxin A (OTA) in nutmeg (Myristicae Semen), ginger (Zingiberis Rhizoma) and turmeric (Curcumae Longae Rhizoma). The matrix matching standard curve was used instead of the standard curve of sample diluent, and the sample extract and sample diluent were optimized. The sensitivity (IC50) of this method for OTA in nutmeg, ginger and turmeric were determined as 0.146, 0.157 and 0.153 ng·mL−1, respectively and the limits of detection (LODs) were 0.040, 0.032 and 0.031 ng·mL−1, respectively. The recoveries of samples ranged from 75.99% to 122.3%, with RSD < 10%. Two positive samples for nutmeg and one positive sample for turmeric occurred in 50 samples, and the highest OTA contamination value was 1 167.8 μg·kg−1. The results were further confirmed by LC-MS/MS. It shows that the developed ic-ELISA method is simple, rapid and sensitive, and can be applied for rapid and high-throughput screening of OTA in nutmeg, ginger and turmeric, as well as some other Chinese herbal medicines.
【Keywords】 indirect competitive enzyme-linked immunosorbent assay; Myristicae Semen; Zingiberis Rhizoma; Curcumae Longae Rhizoma; ochratoxin A; rapid screening;
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