Quality evaluation of Psidii Guajavae Folium from different areas based on fingerprints and stoichiometry

Jul. 5,2022
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Psidii Guajavae Folium (PGF), dried leaf of Psidium guajava Linn. (Myrtaceae), is mainly distributed in Guangdong, Guangxi, Sichuan, Yunnan, Fujian, and Taiwan. PGF has the effects of drying dampness and invigorating spleen, clearing heat and removing toxin, astringing intestines and checking diarrhea, anti-inflammation and stopping bleeding. The main active components of PGF include flavonoids (guaijaverin, isoquercitroside, hyperoside, reynoutrin, avicularin, quercetin) and triterpenoids, which have anti-hyperglycemic, antioxidant, and antibacterial effects. Currently, although PGF has not been included in Chinese Pharmacopoeia (2020 Edition), it has been inscribed on Standards of Guangxi Chinese Materia Medica (1990 Edition), Guangdong Standard for Traditional Chinese Medicinal Materials: Vol Ⅰ (2004 Edition), etc. In the above standards, only quercetin in PGF has been quantified by thin layer chromatography (TLC). As for the quality of PGF, most studies only focus on the content of major components and multi-component quantification, which fail to comprehensively reflect the quality of PGF. At the same time, although P. guajava, as an important economic fruit tree, boasts rich resources, different places of origin make it difficult to control the quality of PGF. In conclusion, there is a lack of sound quality standard and quality evaluation system for PGF.

Li et al. from State Key Laboratory of Traditional Chinese Medicine Resources of Southwest China, Chengdu University of Traditional Chinese Medicine used the high performance liquid chromatography (HPLC) to evaluate the quality of 31 batches of PGF from different habitats. Similarity evaluation, cluster analysis, principal component analysis, and orthogonal partial least squares-discriminant analysis were adopted to analyze the fingerprints. In this study, the HPLC fingerprints for the chemical components of PGF were established, the content of five chemical components, ellagic acid, hyperoside, isoquercitroside, reynoutrin and guaijaverin, in PGF was determined, and the quality of PGF was influenced by geographical region. In summary, HPLC fingerprint in combination with stoichiometry was simple and accurate, which can serve as a reference for the quality control and evaluation of PGF.

The related research was completed by Li Dan, Feng Jing-wen, Liu You-ping, et al. Results are published in the 33rd volume, 6th issue of Natural Products Research and Development, 2021. The bilingual version has been launched.

Corresponding Author: LI Dan
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CNKI Press Officer: LI Jingjing YANG Na
Email: ljj6806@cnki.net yn6791@cnki.net

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