Protective effects of Aronia melanocarpa anthocyanins on oxidative stress and apoptosis in SH-SY5Y cells

Aug. 9,2022
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Currently, some research progress has been made on the preventive and protective effects of anthocyanin on Alzheimer's disease. Studies have shown that strawberry anthocyanin can reduce the occurrence of oxidative stress and oxidative stress-induced apoptosis, thus providing a protective effect on neural cells; anthocyanins in black soybean is effective in reducing neuronal apoptosis by maintaining Ca2 + homeostasis, and can have a protective effect on neuronal cells. The above studies illustrate that anthocyanin may be a potential drug candidate for neurodegenerative diseases such as AD. In addition, a study found that lingonberry anthocyanin, composed of cyanidin-3-O-galactoside, cyanidin-3-O-glucoside and cyanidin-3-O-arabinoside, protected cardiomyocytes from oxidative stress and damage produced by apoptosis. The content of anthocyanins in Aronia melanocarpa is very high and the structure is simple. It is composed of cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside and cyanidin-3-O xyloside, and high-purity anthocyanins are relatively easier to separate.

Meng et al. from School of Food and Pharmaceutical Engineering, Guiyang College used MTT colorimetric assay, RT-PCR and Western blot to investigate the cytoprotective effects of anthocyanins, including cyanidin-3-O-(galactoside, glucoside, arabinoside, xyloside), from A. melanocarpa against oxidative stress and apoptosis induced by Aβ1-42. The result showed that anthocyanins pretreatment significantly inhibited Aβ1-42-induced increase in intracellular ROS, H2O2, and increased the SOD expression through upregulating the Nrf2, HO-1, NQO-1 expression.Apoptosis was significantly inhibited by anthocyanins via down-regulation of Bax and up-regulation of Bcl-2. It can be concluded that high-purity A. melanocarpa anthocyanins could protect SH-SY5Y cell from Aβ1-42-induced oxidative stress and apoptosis through a Nrf2 mechanism, which is important for the further development of anthocyanin and its related products.

The related research was completed by Meng Ling-shuai, Cao Sen, Peng Lin, et al. Results are published in the 33rd volume, 7th issue of Natural Products Research and Development, 2021. The bilingual version has been launched.

Corresponding Author: MENG Lingshuai
CNKI Press Officer: LI Jingjing YANG Na

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